数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PC635 Sigma-AldrichPhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb

This PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Nibrin (pSer³⁴³) Rabbit pAb

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.
Catalogue Number	PC635
Brand Family	Calbiochem®
Synonyms	Anti-Nibrin (pSer³⁴³) Rabbit pAb

References
References	Gatei, M., et al. 2000. Nat. Genet. 25, 115. Lim, D.S., et al. 2000. Nature 404, 613. Wu, G., et al. 2000. J. Biol. Chem. 275, 30618. Zhong, Q., et al. 1999. Science 285, 747. Carney, J.P., et al. 1998. Cell 93, 477. Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447. Varon, R., et al. 1998. Cell 93, 467.

Product Information
Form	Liquid
Formulation	Serum diluted in PBS.
Positive control	γ-irradiated lymphoblastoid cells
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunoprecipitation
Application Notes	ELISA (1:16,000) Immunoblotting (1:1000) Immunoprecipitation (1 µl/500 µg cell extract)
Application Comments	This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Human
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PC635-50ULCN	04055977207828

Documentation

PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb 分析证书

标题	批号
PC635	输入批号

参考

参考信息概述
Gatei, M., et al. 2000. Nat. Genet. 25, 115. Lim, D.S., et al. 2000. Nature 404, 613. Wu, G., et al. 2000. J. Biol. Chem. 275, 30618. Zhong, Q., et al. 1999. Science 285, 747. Carney, J.P., et al. 1998. Cell 93, 477. Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447. Varon, R., et al. 1998. Cell 93, 467.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	12-October-2007 RFH
Synonyms	Anti-Nibrin (pSer³⁴³) Rabbit pAb
Application	ELISA (1:16,000) Immunoblotting (1:1000) Immunoprecipitation (1 µl/500 µg cell extract)
Description	Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.
Background	Nibrin, a ~95 kDa double stranded DNA repair protein, is mutated in Nijmegen breakage syndrome (NBS). Patients with NBS exhibit chromosomal instability, resulting in hypersensitivity to irradiation and cancer predisposition. Nibrin forms a manganese-sensitive complex with hMre11 and hRAD50 that has single strand DNA endonuclease and exonuclease activities and is likely to be important for DNA excision, repair, and stability. This nibrin/Mre11/RAD50 complex is inhibited by BRCA1. The ATM (ataxia telangiectasia mutated) kinase has been shown to phosphorylate nibrin in an S-phase checkpoint pathway. Nibrin levels are elevated in testis tissue, suggesting a possible role in meiotic recombination. Nibrin may also be involved in telomere length maintenance.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
Isotype	IgG
Species	human
Positive control	γ-irradiated lymphoblastoid cells
Form	Liquid
Formulation	Serum diluted in PBS.
Preservative	None
Comments	This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Gatei, M., et al. 2000. Nat. Genet. 25, 115. Lim, D.S., et al. 2000. Nature 404, 613. Wu, G., et al. 2000. J. Biol. Chem. 275, 30618. Zhong, Q., et al. 1999. Science 285, 747. Carney, J.P., et al. 1998. Cell 93, 477. Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447. Varon, R., et al. 1998. Cell 93, 467.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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