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Recognizes the ~55 kDa c-Fos protein phosphorylated at Ser374. Does not detect the unphosphorylated form.
Catalogue Number
ST1029
Brand Family
Calbiochem®
Application Data
Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
References
References
Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10952.
Product Information
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
Does not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
Immunogen
Human
Clone
34E4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Shipped with Blue Ice or with Dry Ice
Toxicity
Irritant
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号
GTIN
ST1029-1SETCN
04055977208689
Documentation
PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) MSDS
PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) 分析证书
标题
批号
ST1029
参考
参考信息概述
Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10952.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
Description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Supplied with a positive control lysate consisting of EGF-treated HepG2 cells. Recognizes the ~55 kDa c-fos protein phosphorylated at Ser374 by MAP kinase.
Background
The early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at Thr325.
Host
Mouse
Immunogen species
Human
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
Clone
34E4
Isotype
IgG₁
Species
human
Positive control
HepG2 cells treated with EGF
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
Preservative
≤0.1% sodium azide (antibody only)
Comments
Does not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity
Irritant
References
Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10952.
