数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

324867 Sigma-AldrichPhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

This PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ch, H, M, R	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number	324867
Brand Family	Calbiochem®
Synonyms	Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
Application Data	Detection of human EGFR, phosphorylated at Tyr¹⁰⁶⁸, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.

References
References	Jo, M., et al. 2000. J. Biol. Chem. 275, 8806. Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572. Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

References

Jo, M., et al. 2000. J. Biol. Chem. 275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

Product Information
Form	Liquid
Formulation	In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting)
Application Notes	Immunoblotting (1:1000)
Application Comments	When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Chicken Human Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
324867-10TCN	04055977215823

Documentation

PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb 分析证书

标题	批号
324867	输入批号

参考

参考信息概述
Jo, M., et al. 2000. J. Biol. Chem. 275, 8806. Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572. Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	03-March-2008 JSW
Synonyms	Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
Application	Immunoblotting (1:1000)
Application Data	Detection of human EGFR, phosphorylated at Tyr¹⁰⁶⁸, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
Description	Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸.
Background	Epidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domains. Upon binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domains display tyrosine kinnase activity. This leads to autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as phosphorylation of intracellular substrates. Tyr¹⁰⁶⁸ is an autophosphorylation site in EGFR. The SH2 domain of Grb2 binds to Tyr¹⁰⁶⁸ of EGFR in the multimeric complex EGFR-Grb2-mSos1 that is formed upon stimulation of EGFR with EGF.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
Isotype	IgG
Species	chicken, human, mouse, rat
Form	Liquid
Formulation	In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
Preservative	≤0.1% sodium azide
Comments	When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
References	Jo, M., et al. 2000. J. Biol. Chem. 275, 8806. Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572. Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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