Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Pellet Paint NF Co-Precipitant is a
nonfluorescent dye-labeled carrier compatible
with fluorescent sequencing. It facilitates
rapid removal of BigDye Terminators during
the alcohol precipitation of cycle sequencing
reaction products. Cycle sequencing reactions
can be precipitated rapidly with 1 µl of carrier
per reaction and centrifugation times of 10
minutes. The easily visualized carrier provides
a simple confirmation that precipitation
has occurred. Sequencing reaction products
are efficiently pelleted and dye-labeled
terminators remain in the supernatant during
alcohol precipitation using the standard
Applied Biosystems precipitation protocols.
Resuspension of pelleted sequencing reaction
products in deionized formamide can be
confirmed by checking for dissolution of
the carrier. Pellet Paint NF Co-Precipitant
is fully compatible with the ABI PRISM®
BigDye Terminator Cycle Sequencing Ready
Reaction. To avoid extra sample handling,
Pellet Paint NF Co-Precipitant can be added
directly to the reaction mix, template DNA,
crude PCR samples, or dilution buffer before
the cycle sequencing reaction. Although Pellet
Paint NF absorbs in the UV range, accurate
spectrophotometric measurements of DNA or
RNA samples are possible; the absorbance ratio
(provided with each package of Pellet Paint
NF) can be used as a correction factor when
determining nucleic acid concentration. Pellet
Paint NF Co-Precipitant has no detectable
effect on the sequencing reaction or sequence
accuracy. Pellet Paint NF Co-Precipitant is
a useful substitute for the original Pellet
Paint Co-Precipitant in applications where
fluorescent detection is used.
Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending
Catalogue Number
70748
Brand Family
Novagen®
Features and benefits
Efficient and rapid precipitation of BigDye® cycle sequencing products
Efficient removal of dye terminators
Direct visualization and tracking of precipitated material
No effect on sequencing reaction
Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
References
Product Information
•
125 µl or 1 ml
Pellet Paint NF Co-Precipitant
•
1 ml or 8 ml
3 M Sodium Acetate, pH 5.2
Declaration
This product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates.
Taku Nakagawa, et al. (2007) Detection of circulating tumor cells in early-stage breast cancer metastasis to axillary lymph nodes. Clinical Cancer Research13, 4105-4110.
