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A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number
53702
Brand Family
Calbiochem®
References
References
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
Product Information
CAS number
9036-06-0
Activity
≥45,000 proteolytic units/g dry weight
Unit of Definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Form
Lyophilized
Formulation
Lyophilized from a solution containing calcium acetate as a stabilizer.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Description
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Form
Lyophilized
Formulation
Lyophilized from a solution containing calcium acetate as a stabilizer.
CAS number
9036-06-0
RTECS
UK9595000
Activity
≥45,000 proteolytic units/g dry weight
Unit definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Solubility
H₂O (10 mg/ml)
Storage
+2°C to +8°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
