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Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
Note: 4µg = 6000 units.
Catalogue Number
539578
Brand Family
Calbiochem®
Synonyms
cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
Application Data
References
References
Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95. Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
Form
Liquid
Formulation
In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95. Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Synonyms
cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
Application Data
Description
Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
Form
Liquid
Formulation
In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
Concentration Label
Please refer to vial label for lot-specific concentration
Specific activity
≥1300 units/µg enzyme
Unit definition
One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95. Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.
