数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

539672 Sigma-AldrichPKCδ, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Protein Kinase Cδ

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概述

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Description
Overview	Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca²⁺-independent and phospholipid-dependent enzyme.
Catalogue Number	539672
Brand Family	Calbiochem®
Synonyms	Protein Kinase Cδ

References
References	Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329. Kazanietz, M.G., et al. 1993. Mol. Pharmacol. 44, 298. Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.

Product Information
Unit of Definition	One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKC<sub>ε</sub> Peptide Substrate (<a href ="/Products/ProductDisplay.asp→catNO=539562">Cat. No. 539562</a>) per min at 30°C, pH 7.5.
Form	Liquid
Formulation	In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^® X-100 Detergent, pH 7.5.
Quality Level	MQ100

Applications

Biological Information
Purity	≥95% by SDS-PAGE
Specific Activity	≥800 units/mg protein
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
539672-5UGCN	07790788051358

Documentation

PKCδ, Human, Recombinant, S. frugiperda MSDS

职位
物料安全数据表 (MSDS)

PKCδ, Human, Recombinant, S. frugiperda 分析证书

标题	批号
539672	输入批号

参考

参考信息概述
Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329. Kazanietz, M.G., et al. 1993. Mol. Pharmacol. 44, 298. Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	08-September-2008 RFH
Synonyms	Protein Kinase Cδ
Description	Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca²⁺-independent and phospholipid-dependent enzyme.
Form	Liquid
Formulation	In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^® X-100 Detergent, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Recommended reaction conditions	Protocol for Recombinant PKC δ Note: this preparation is purified to near homogeneity, so it may behave differently than crude preparations. In particular, the concentration and quality of the phospholipids used does not seem to be important. Lipid Mixture: 1. Determine the total amount of lipid mixture needed. Each reaction requires 20 µg of phosphatidylserine (1.2 µl of 10 mg/ml phosphatidylserine in CHCl₃) and 2 mg of diacylglycerol (0.6 µl of 2 mg/ml diacylglycerol in CHCl₃). Preparing 10% more lipid mixture than required is recommended to account for losses while pipetting. 2. Using a Hamilton syringe that has been cleaned with methanol, transfer the required volume of each lipid stock solution to a 12 x 75 mm glass tube. 3. Evaporate off the chloroform. 4. Resuspend in 10 µl of 10 mM HEPES, pH 7.4, 0.3% TRITON^® X-100 detergent. 5. Vortex for at least 2 min. 6. Place the lipid mixture in a 40°C water bath for 5 min prior to adding the reaction mixture. Reaction Mixture: 2.4 µl 500 mM HEPES, pH 7.4 6 µl 100 mM MgCl2 6 µl 1 mM EGTA 6 µl 1 mg/ml PKC ε peptide (Cat. No. 539562) 0.6 µl 10 mM ATP 6 µl lipid mixture 0.1 µl γ-ATP (5 mCi/ml) 32.9 µl distilled H₂O Total volume should be 60 µl. Prepare a sufficient reaction mixture for all assays to be performed. Reaction: 1. Dispense 60 µl of reaction mixture into each tube and incubate at 30°C. 2. Dilute the PKC δ stock solution (500 ng/µl) to between 20-50 ng/µl in 10 mM Tris-HCl, pH 7.5, 5 mM DTT, and 0.01% TRITON^®: X-100 detergent. 3. Add 2 µl of diluted enzyme to each tube. 4. Incubate for 10 min. 5. Stop the reaction by adding 6 µl of 5% phosphoric acid. 6. Incubate on ice for 5 min. 7. Spot 55 µl from each tube to phosphocellulose membranes and allow to dry. 8. Wash the membranes 3X with 5 ml of 0.5% phosphoric acid per filter. 9. Transfer membranes to a scintillation vial and count. Note: This protocol is meant to serve as a guideline and may need to be modified for specific applications.
Purity	≥95% by SDS-PAGE
Specific activity	≥800 units/mg protein
Unit definition	One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKC_ε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5.
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
References	Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329. Kazanietz, M.G., et al. 1993. Mol. Pharmacol. 44, 298. Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.

种类

Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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