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Recombinant, human P-selectin consisting of 730 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is expressed on activated platelets and endothelial cells. A specific interaction between P-selectin and P-selectin glycoprotein ligand-1, present on numerous cell types, promotes adhesion and acts along with L-selectin in establishing an initial interaction between circulating leukocytes and the endothelium.
Catalogue Number
561306
Brand Family
Calbiochem®
Synonyms
CD62P, GMP-140, LECAM-3, PADGEM
References
Product Information
Form
Lyophilized
Formulation
Lyophilized from Dulbecco’s D-PBS (containing calcium and magnesium), 1% sorbitol.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-September-2008 RFH
Synonyms
CD62P, GMP-140, LECAM-3, PADGEM
Description
Recombinant, human P-selectin consisting of 730 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is expressed on activated platelets and endothelial cells. A specific interaction between P-selectin and P-selectin glycoprotein ligand-1, present on numerous cell types, promotes adhesion and acts along with L-selectin in establishing an initial interaction between circulating leukocytes and the endothelium.
Form
Lyophilized
Formulation
Lyophilized from Dulbecco’s D-PBS (containing calcium and magnesium), 1% sorbitol.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxins: <1.5 EU/µg of P-Selectin
Biological activity
Shown to bind to P-selectin ligands on U937 cells.
Solubility
Reconstitute at 1 mg/ml in sterile H₂O.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.