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Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
M.W. 65,000 and 30,000 under reducing conditions.
Catalogue Number
499260
Brand Family
Calbiochem®
Synonyms
rmOPN
References
References
Xie, Y., et al. 2001. Kidney Int.60, 1645. Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319. Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
Product Information
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
ED50 = 0.25-1.5 µg/ml based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxins: ≤100 pg/µg rmOPN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
Xie, Y., et al. 2001. Kidney Int.60, 1645. Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319. Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-June-2019 JSW
Synonyms
rmOPN
Description
Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxins: ≤100 pg/µg rmOPN
Biological activity
ED50 = 0.25-1.5 µg/ml based on the addition of 1 x 10⊃5 cells per well coated with osteopontin and incubated for 1 hr at 37°C.
Solubility
Reconstitute to ≥100 µg/ml with sterile PBS.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
Toxicity
Standard Handling
References
Xie, Y., et al. 2001. Kidney Int.60, 1645. Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319. Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
