Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
Catalogue Number
492899
Brand Family
Calbiochem®
References
References
Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.
Product Information
CAS number
9013-53-0
Unit of Definition
One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
15-August-2019 JSW
Description
Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
Form
Lyophilized white to off-white solid
CAS number
9013-53-0
EC number
3.1.31.1
Specific activity
≥15,000 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
Solubility
Aqueous buffers (1 mg/ml)
Storage
+2°C to +8°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.
