Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Product Description
The NovaQUANT™ Mouse Mitochondrial to Nuclear DNA Ratio Kit compares the levels of nuclear to mitochondrial DNA (mtDNA) in a mouse DNA sample. Real-Time PCR provides a platform for a quantitative assay measuring and comparing mtDNA copy number to that of nuclear DNA (nDNA). This mtDNA/nDNA ratio test kit in qPCR format can be used whenever a small amount of DNA can be extracted from cells or tissues of interest. The amplification primers in this kit have been thoroughly validated for matching amplification efficiency and absence of amplification of mitochondrial pseudogenes.
This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes. A single assay is run using four wells, whereby each well individually tests for one of four gene targets (see Figure 1 at the bottom of this page). Resultant Cts obtained from the Real-Time instrument are used to represent the level of each gene. Nuclear gene 1 is compared to that of mitochondrial gene 1 and nuclear gene 2 to mitochondrial gene 2. In this way, ratios are calculated which can be averaged to represent the mtDNA copy number per cell.
Panel Components
• 2 ea Mouse qPCR plate with pre-aliquoted PCR primer pairs
• 100 ng Mouse total DNA (10 ng/µl)
• 100 ng Mouse rho zero DNA (10 ng/µl)
Storage: Store all components at 4 °C for up to three months; we recommend storing the control DNA at –20 °C for long term storage.
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