Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
The Ni-NTA Buffer Kit provides a convenient set of buffers optimized for purification of His•Tag fusion proteins on Ni-NTA His•Bind Resin. These phosphate-buffered solutions differ from the Tris-based solutions used in the His•Bind Buffer Kit. Carefully prepared 4X concentrates are included for binding, washing and elution according to recommended protocols.
Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics6, 189-194.