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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

362300 Sigma-AldrichN-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F

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概述

Replacement Information

价格及供货情况

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Description
Overview	Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides.
Overview	Note: 1 mU = 1 milliunit.
Catalogue Number	362300
Brand Family	Calbiochem®
Synonyms	Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F

References
References	Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058. Altmann, F., et al. 1995. Glycoconj. J. 12, 84.

Product Information
CAS number	83534-39-8
Unit of Definition	One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.
EC number	3.5.1.52
Form	Liquid
Formulation	Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Quality Level	MQ100

Applications

Biological Information
Specific Activity	≥10 units/mg protein

Physicochemical Information
Contaminants	β-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases: none detected

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
362300-1SETCN	04055977213669

Documentation

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli MSDS

职位
物料安全数据表 (MSDS)

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli 分析证书

标题	批号
362300	输入批号

参考

参考信息概述
Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058. Altmann, F., et al. 1995. Glycoconj. J. 12, 84.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-March-2019 JSW
Synonyms	Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F
Description	Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.
Form	Liquid
Formulation	Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Recommended reaction conditions	• Reaction Buffer (Component No. KP31859-1EA): 1 vial, 1 ml, 100 mM sodium phosphate, 0.1% sodium azide, pH 7.5, supplied as a 5X solution • Denaturation Solution (Component No. KP31860-1EA): 1 vial, 200 µl, 2% SDS, 1 M β-mercaptoethanol • Detergent Solution (Component No. KP31861-1EA): 1 vial, 200 µl, 15% NP-40 solution • Tris Reaction Buffer (Component No. KP31865-1EA): 1 vial, 1 ml, 50 mM Tris-HCl, pH 8.0 *• N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli* (Component No. KP31858-100MIU): 1 vial, 100 mU Note: Tris Reaction Buffer has been included as an alternative reaction buffer because phosphate buffers should be avoided if mass spectrometry is used in downstream analysis. Procedure for Deglycosylation (Denaturing Conditions)** The amount of enzyme required for deglycosylation depends on the substrate used, the incubation conditions, and the exact application. This protocol is provided only as a guide. Assay conditions should be adjusted as required for individual applications. 1. Prepare 50 to 500 µg glycoprotein solution in 45 µl 1X Reaction. Add 2.5 µl Denaturation Solution. 2. Denature glycoprotein by heating at 100°C for 5 min. Allow mixture to cool. 3. Add 2.5 µl Detergent Solution. 4. Add 2 µl N-Glycosidase to the reaction mixture and incubate for 2 h to overnight at 37°C.
CAS number	83534-39-8
EC number	3.5.1.52
Contaminants	β-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases: none detected
Specific activity	≥10 units/mg protein
Unit definition	One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058. Altmann, F., et al. 1995. Glycoconj. J. 12, 84.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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