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Sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA2 in endothelial cells.
Catalogue Number
34115
Brand Family
Calbiochem®
Synonyms
NEM
References
References
Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485. Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57. Cain, K., et al. 1994. Biochem. Cell Biol.72, 631. Banger, K.K., et al. 1993. Biochem. J. 290, 199. Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.
Harmful in contact with skin. Very toxic if swallowed. Causes burns. May cause sensitization by skin contact.
S Phrase
S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Corrosive
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-February-2014 JSW
Synonyms
NEM
Description
Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei.
Form
White solid
CAS number
128-53-0
RTECS
UX9625000
Chemical formula
C₆H₇NO₂
Structure formula
Purity
≥98% by HPLC
Solubility
Ethanol (5 mg/ml)
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Corrosive
Merck USA index
14, 3822
References
Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485. Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57. Cain, K., et al. 1994. Biochem. Cell Biol.72, 631. Banger, K.K., et al. 1993. Biochem. J. 290, 199. Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.
