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Native, yeast myokinase. Myokinase reversibly transfers phosphate from ATP to AMP, thus forming two molecules of ADP. The enzyme is specific for adenine nucleotides and catalyzes the reaction only in the presence of a divalent metal ion (e.g. Mg2+, Ca2+, Co2+, Mn2+, or Ni2+).
Note: 1 KU = 1000 units.
Catalogue Number
475941
Brand Family
Calbiochem®
Synonyms
Adenylate Kinase, ATP:AMP Phosphotransferase
References
References
Makarchikov, A.F., et al. 2002. Biochem. Biophys. Acta.1592, 117. Schricker, R., et al. 2002. J. Biol. Chem.277, 28757. Magdolen, V., et al. 1987. Curr. Genet.12, 405. Tomasselli, A.G., et al. 1986. Eur. J. Biochem.155, 111. Ito, Y., et al. 1980. Eur. J. Biochem.105, 85.
Product Information
CAS number
9013-02-9
Activity
≥10 units/mg solid
Unit of Definition
One unit is defined as the amount of enzyme that will convert 1.0 µmol ATP and 1.0 µmol AMP to 2 µmol ADP per min at 25°C, pH 7.5. Note: 1 KU = 1000 units.
Makarchikov, A.F., et al. 2002. Biochem. Biophys. Acta.1592, 117. Schricker, R., et al. 2002. J. Biol. Chem.277, 28757. Magdolen, V., et al. 1987. Curr. Genet.12, 405. Tomasselli, A.G., et al. 1986. Eur. J. Biochem.155, 111. Ito, Y., et al. 1980. Eur. J. Biochem.105, 85.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Synonyms
Adenylate Kinase, ATP:AMP Phosphotransferase
Description
Native, yeast myokinase. Myokinase reversibly transfers phosphate from ATP to AMP, thus forming two molecules of ADP. The enzyme is specific for adenine nucleotides and catalyzes the reaction only in the presence of a divalent metal ion (e.g. Mg2+, Ca2+, Co2+, Mn2+, or Ni2+).
Form
Lyophilized
Formulation
Lyophilized from potassium phosphate buffer.
CAS number
9013-02-9
EC number
2.7.4.3
Contaminants
ATPase: ≤0.01%; Phosphoglycerate kinase: ≤0.1%
Specific activity
≥200 units/mg
Activity
≥10 units/mg solid
Unit definition
One unit is defined as the amount of enzyme that will convert 1.0 µmol ATP and 1.0 µmol AMP to 2 µmol ADP per min at 25°C, pH 7.5. Note: 1 KU = 1000 units.
Solubility
H₂O (1 mg/ml)
Storage
-20°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Makarchikov, A.F., et al. 2002. Biochem. Biophys. Acta.1592, 117. Schricker, R., et al. 2002. J. Biol. Chem.277, 28757. Magdolen, V., et al. 1987. Curr. Genet.12, 405. Tomasselli, A.G., et al. 1986. Eur. J. Biochem.155, 111. Ito, Y., et al. 1980. Eur. J. Biochem.105, 85.