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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

444213 Sigma-AldrichMMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase

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Description
Overview	Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.
Overview	Note: 1 mU = 1 milliunit.
Catalogue Number	444213
Brand Family	Calbiochem®
Synonyms	Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase

References
References	Buisson-Leqendre, N., et al. 2004. Arth. Rheumat. 50, 2151. Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7414. Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

Product Information
Unit of Definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
EC number	3.4.24.24
Form	Liquid
Formulation	In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.
Quality Level	MQ100

Applications

Biological Information
Purity	≥90% by SDS-PAGE
Source	Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity	≥600 mU/mg protein
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information
Contaminants	no other MMP activity detectable

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
444213-5UGCN	04055977205190

Documentation

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast MSDS

职位
物料安全数据表 (MSDS)

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast 分析证书

标题	批号
444213	输入批号

参考

参考信息概述
Buisson-Leqendre, N., et al. 2004. Arth. Rheumat. 50, 2151. Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7414. Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

引用

标题

Feng Zheng, et al. (2004) Development of albuinuria and glomerular lesions in normoglycemic B6 recepients of db/db mice bone marrow. Diabetes 53, 2420-2427.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	18-July-2007 RFH
Synonyms	Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
Description	Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.
Form	Liquid
Formulation	In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.
Concentration Label	Please refer to vial label for lot-specific concentration
Recommended reaction conditions	Organomercurial Activation Protocol This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. The following protocol is from Stricklin, et al., which describes the use of p-aminophenylmercuric acetate (APMA) to activate pro-MMP. This protocol is also adaptable to other types of organomercurals, such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), or mersalyl. 1. Prepare a 10-50 mM stock solution of APMA (or other organomercurial compound) in 0.1 M NaOH just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl (see Marcy, A.I., et al.). 2. To initiate the activation mix the proenzyme solution with the APMA solution at a 10:1 volume ratio (MMP:APMA). If a higher concentration of APMA is desired, increase the concentration of the stock solution. Do not exceed the 10:1 ratio, as this could result in significant changes in pH. 3. Incubate the mixture at 37°C for 2-3 h. It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with a fixed concentration of pro-MMP and organomercurial would be incubated as described above. Remove aliquots of the sample at various time points during the incubation. Stop the reaction by the addition of SDS-PAGE sample buffer (e.g., 10 µl 2X sample buffer to 10 µl aliquot) and heat the samples to 95°C. The progress of activation can be monitored qualitatively by analyzing the aliquots on a 12% SDS-PAGE gel. 4. The activated MMP can be used without removing the APMA from the mixture. Please refer to Marcy, A.I., et al. for removal of organomercurials by gel filtration.
Source	Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
EC number	3.4.24.24
Purity	≥90% by SDS-PAGE
Contaminants	no other MMP activity detectable
Specific activity	≥600 mU/mg protein
Unit definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
Solubility	Aqueous buffers
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
References	Buisson-Leqendre, N., et al. 2004. Arth. Rheumat. 50, 2151. Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7414. Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
Citation	Feng Zheng, et al. (2004) Development of albuinuria and glomerular lesions in normoglycemic B6 recepients of db/db mice bone marrow. Diabetes 53, 2420-2427.

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