Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recombinant, human MMP-13. MMP-13 is a collagenase-3 enzyme that degrades a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X, and XIV, gelatin, aggrecan, perlecan and fibronectin. It is distinguished from other human collagenases by the fact that it effectively degrades type II collagen. The importance of MMP-13 is further strengthened by reports that it is a biomarker for poor prognosis in many carcinomas and other type of cancers.
Catalogue Number
444287
Brand Family
Calbiochem®
References
References
Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.
Product Information
Unit of Definition
One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1µmol MCA-Pro-Leu-Lys-Gly-Leu-DPA-Ala-Arg-NH₂ (Cat. No. 03-32-5032) at 37°C, pH 7.5.
Form
Liquid
Formulation
In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ® 35 Detergent, pH 7.5. APMA-free.
Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-March-2009 JSW
Description
Recombinant, human MMP-13. MMP-13 is a collagenase-3 enzyme that degrades a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X, and XIV, gelatin, aggrecan, perlecan and fibronectin. It is distinguished from other human collagenases by the fact that it effectively degrades type II collagen. The importance of MMP-13 is further strengthened by reports that it is a biomarker for poor prognosis in many carcinomas and other type of cancers.
Form
Liquid
Formulation
In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ® 35 Detergent, pH 7.5. APMA-free.
Concentration Label
Please refer to vial label for lot-specific concentration
Specific activity
≥50 mU/mg protein
Unit definition
One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1µmol MCA-Pro-Leu-Lys-Gly-Leu-DPA-Ala-Arg-NH₂ (Cat. No. 03-32-5032) at 37°C, pH 7.5.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.
