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HSTCMAG28SPMX21 MilliporeMILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
小册子
技术信息
Protocols

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease

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概述

Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α	H	Mplex	Luminex xMAP

Description
Catalogue Number	HSTCMAG28SPMX21
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay
Background Information	Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

References

Product Information
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Inter-assay: <15% Intra-assay: <10%
Panel Type	MAGNETIC Cytokines/Chemokines
Quality Level	MQ200

Applications
Application	Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight incubation. • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. These features are summarized on the next page. • This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.

Biological Information
Species Reactivity	Human
Analytes Available	Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α
Cross Reactivity	There is no detectable cross-reactivity within the panel.
Accuracy	98% – 107%

Physicochemical Information
Sensitivity	0.11 - 8.17 pg/mL (Overnight Incubation minDC) Please see kit protocol for individual assay sensitivities.
Linearity of Dilution	Please contact technical service for information
Standard Curve Range	Fractalkine: 18.3 – 75,000 pg/mL GM-CSF: 1.22 – 5,000 pg/mL IFNγ: 0.61 – 2,500 pg/mL IL-1β: 0.49 – 2,000 pg/mL IL-2: 0.49 – 2,000 pg/mL IL-4: 1.83 – 7,500 pg/mL IL-5: 0.49 – 2,000 pg/mL IL-6: 0.18 – 750 pg/mL IL-7: 0.37 – 1,500 pg/mL IL-8: 0.31 – 1,250 pg/mL IL-10: 1.46 – 6,000 pg/mL IL-12 (p70): 0.49 – 2,000 pg/mL IL-13: 0.24 – 1,000 pg/mL IL-17A: 0.73 – 3,000 pg/mL IL-21: 0.24 – 1,000 pg/mL IL-23: 7.93 – 32,500 pg/mL ITAC: 1.46 – 6,000 pg/mL MIP-1α: 0.31 – 1,250 pg/mL MIP-1β: 0.92 – 3,750 pg/mL MIP-3α: 0.61 – 2,500 pg/mL TNFα: 0.45 – 1,750 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
HSTCMAG28SPMX21	04055977289114

Documentation

Protocols

Title
Protocol- HSTCMAG-28SK\|HSTCMAG28SPMX13\|HSTCMAG28SPMX21\|HSTCMAG28PMX13BK\|HSTCMAG28PMX21BK

MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 21-plex - Immunology Multiplex Assay MSDS

职位
物料安全数据表 (MSDS)

小册子

标题
The Power of Biomarker Analysis

技术信息

标题
96 well Template 2 Up
96 well Template Single
MILLIPLEX MAP Human High Sensitivity T Cell Panel
Quality Control Range Sheet- HST-111 and HST-211
Quality Control Ranges: Milliplex Human High Sensitivity T Cell Magnetic Bead Panel

相关产品和应用

种类

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Premixed Kits

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