Millipore Sigma Vibrant Logo

454850 MAP Kinase 2, Mouse, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: ERK2, MAPK2, p42MAPK

View Products on Sigmaaldrich.com
454850
查看价格及供货情况

概述

Replacement Information

价格及供货情况

产品目录编号 库存情况包装 数量 / 包装 价格 数量
454850-2000UCN
正在查询供货情况......
—
目前缺货 目前缺货
有货 
停产
少量现货
供货情况有待确认
    其余：另行通知
      其余：另行通知
      另行通知
      联系客户服务
      Contact Customer Service

      		 塑胶安瓿;塑胶针药瓶 2000 u
      正在检索价格......
      无法检索价格。
      最小订购数量为倍数
      Maximum Quantity is
      以订单确认为准 更多信息
      您节约了 ()。
       
      —
      Request Pricing
      		 —
      Description
      OverviewRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
      Catalogue Number454850
      Brand Family Calbiochem®
      SynonymsERK2, MAPK2, p42MAPK
      References
      ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.
      Product Information
      Activity≥5000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      ContaminantsMEK, phosphatase, protease: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      454850-2000UCN 07790788050399

      Documentation

      MAP Kinase 2, Mouse, Recombinant, E. coli MSDS

      职位

      物料安全数据表 (MSDS) 

      MAP Kinase 2, Mouse, Recombinant, E. coli 分析证书

      标题批号
      454850

      参考

      参考信息概述
      Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.
      数据表

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2008 RFH
      SynonymsERK2, MAPK2, p42MAPK
      DescriptionRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
      Recommended reaction conditions
      Note: This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. Suggested Protocol to Assay for MAPK Activators 1. Combine the following on ice: 4.0 µl 250 mM HEPES, pH 7.5, 100 mM Mg(OAc)2, 500 µM ATP 2.0 µl MAP Kinase 1-29 µl purified MEK (cell lysates or fractions from chromatography) 2.0 µl [γ-32P]ATP (1.0 µCi/µl) add enough distilled H2O to produce a final volume of 40 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by adding 40 µl of 2X SDS-PAGE sample buffer. 4. The phosphorylated MAPK will show as a signal at ~45 kDa by autoradiography. Phosphorylating Protein Substrates with Phosphorylated MAPK 1. Combine the following on ice: 5.0 µl 500 mM Tris-HCl (pH 7.5), 62.5 mM β-glycerol phosphate, 50 mM MgCl2, 5 mM EGTA, 250 µM NaF, 5 mM DTT, and 2.5 mM sodium vanadate 2 µg experimental protein or Myelin Basic Protein Peptide Substrate (Cat. No. 475920) 1.0 µl phosphorylated MAPK 2.0 µl [γ-32P]ATP (1.25 mM,100 µCi/µmoll) add enough distilled H2O to produce a final volume of 25 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by spotting 20 µl of each reaction mixture onto a 1.5 cm2 piece of Whatman P81 paper. 4. Wash the squares 7X for 5 min with phosphoric acid (1% w/v). 5. Quantitate the 32P incorporation in a liquid scintillation counter.
      Purity≥90% by SDS-PAGE
      ContaminantsMEK, phosphatase, protease: none detected.
      Activity≥5000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.