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Recombinant, human MAP Kinase 1 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Catalogue Number
454849
Brand Family
Calbiochem®
Synonyms
ERK1, MAPK3, p44MAPK
References
References
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli 分析证书
标题
批号
454849
参考
参考信息概述
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-August-2008 RFH
Synonyms
ERK1, MAPK3, p44MAPK
Description
Recombinant, human MAP Kinase 1 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Purity
≥95% by SDS-PAGE
Contaminants
Proteases: none detected
Specific activity
≥6000 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.