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Native lysozyme from human neutrophils. Occurs in specific granules of human polymorphonuclear leukocytes and takes part in antibacterial activity of leukocytes. Hydrolyzes β1,4 linkages between N-acetylmuramic acid (2-acetamido-2-deoxy-3-O-[1′-carboxymethyl]-glucopyranose) residues in chitin.
Catalogue Number
440345
Brand Family
Calbiochem®
Synonyms
N-Acetylmuramyl Hydrolase, Muramidase
References
References
Lollike, K., et al. 1995. Leukemia 9, 206. Gordon, L.I., et al. 1979. J. Clin. Invest. 64, 226.
Product Information
CAS number
9001-63-2
Unit of Definition
One Shugar unit is defined as the amount of enzyme that will cause a decrease in absorbance of 0.001 at 450 nm per min due to lysis of <i>Micrococcus lysodeikticus</i> at 37°C, pH 7.0.
EC number
3.2.1.17
Form
Lyophilized
Formulation
Lyophilized from 100 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Applications
Biological Information
Purity
≥95% by SDS-PAGE
Source
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity
≥ 30,000 shugar units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
OL5987000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 year at -20°C.
Lollike, K., et al. 1995. Leukemia 9, 206. Gordon, L.I., et al. 1979. J. Clin. Invest. 64, 226.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-November-2013 JSW
Synonyms
N-Acetylmuramyl Hydrolase, Muramidase
Description
Native lysozyme from human neutrophils. Occurs in specific granules of human polymorphonuclear leukocytes and takes part in antibacterial activity of leukocytes. Hydrolyzes β1,4-linkages between N-acetylmuramic acid (2-acetamido-2-deoxy-3-O-[1'-carboxymethyl]-glucopyranose) residues in chitin.
Form
Lyophilized
Formulation
Lyophilized from 100 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Source
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number
9001-63-2
RTECS
OL5987000
EC number
3.2.1.17
Purity
≥95% by SDS-PAGE
Specific activity
≥ 30,000 shugar units/mg protein
Unit definition
One Shugar unit is defined as the amount of enzyme that will cause a decrease in absorbance of 0.001 at 450 nm per min due to lysis of Micrococcus lysodeikticus at 37°C, pH 7.0.
Solubility
Aqueous Buffers or H₂O
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 year at -20°C.
Toxicity
Standard Handling
Merck USA index
14, 5640
References
Lollike, K., et al. 1995. Leukemia 9, 206. Gordon, L.I., et al. 1979. J. Clin. Invest. 64, 226.