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Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Catalogue Number
440319
Brand Family
Calbiochem®
References
References
D'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266. Nikitovic, D. et al. 2008. IUBMB Life60, 818. Nikitovic, D. et al. 2008. FEBS J.275, 350. Ishiwata, T. et al. 2007. Oncol. Rep.18, 537. Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294. Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422. Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532. Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365. Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347. Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
D'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266. Nikitovic, D. et al. 2008. IUBMB Life60, 818. Nikitovic, D. et al. 2008. FEBS J.275, 350. Ishiwata, T. et al. 2007. Oncol. Rep.18, 537. Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294. Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422. Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532. Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365. Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347. Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-June-2011 RFH
Description
Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered PBS.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Biological activity
Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
Solubility
Reconstitute to 200 µg/ml with sterile PBS.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
D'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266. Nikitovic, D. et al. 2008. IUBMB Life60, 818. Nikitovic, D. et al. 2008. FEBS J.275, 350. Ishiwata, T. et al. 2007. Oncol. Rep.18, 537. Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294. Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422. Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532. Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365. Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347. Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
