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A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number
530650
Brand Family
Calbiochem®
References
References
Evans, M., et al. 2014. Nat Methods.11, 393. Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.
Evans, M., et al. 2014. Nat Methods.11, 393. Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
15-April-2019 JSW
Description
A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.
Form
Yellow powder
Intert gas (Yes/No)
Packaged under inert gas
Chemical formula
C₁₃H₁₁N₃O₂S₂
Purity
≥94% by HPLC
Solubility
DMSO (100 mg/ml)
Storage
Protect from light
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Evans, M., et al. 2014. Nat Methods.11, 393. Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.
