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Native lactate dehydrogenase from rabbit muscle. Catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation.
Note: 1 KU = 1000 units.
Catalogue Number
427217
Brand Family
Calbiochem®
Synonyms
LDH
References
References
Cahn, R.C., et al. 1962. Science136, 962.
Product Information
CAS number
9001-60-9
Unit of Definition
One unit is defined as the amount of enzyme that will convert 1.0 µmol pyruvate to L-lactate per min in 100 mM phosphate buffer at 30°C, pH 7.0.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-May-2008 RFH
Synonyms
LDH
Description
Native lactate dehydrogenase from rabbit muscle. Catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH 6.0.
Form
Lyophilized
CAS number
9001-60-9
EC number
1.1.1.27
Contaminants
GOT and GPT: <0.005%
Specific activity
≥200 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will convert 1.0 µmol pyruvate to L-lactate per min in 100 mM phosphate buffer at 30°C, pH 7.0.
Solubility
H₂O or Low Ionic Strength Buffer
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.