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Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units
Catalogue Number
427211
Brand Family
Calbiochem®
References
References
Kornberg, A. 1995. Methods Enzymol.1, 441
Product Information
CAS number
9001-60-9
Unit of Definition
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
24-August-2009 JSW
Description
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
Form
Lyophilized
CAS number
9001-60-9
EC number
1.1.1.27
Contaminants
GPT, GOT: ≤0.03%; PK, MK: ≤0.01%; MDH: ≤0.1%
Specific activity
≥300 units/mg protein. One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
Unit definition
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
Solubility
Aqueous Buffers
Storage
Protect from moisture
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
