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Recombinant, human, interleukin-6 expressed in E. coli. A prototypic pleiotrophic, pro-inflammatory cytokine that stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is produced by macrophages, endothelial cells, and activated T helper cells. Also considered as a potential marker of adipocyte differentiation. Human IL-6 is active on murine cells.
Catalogue Number
407652
Brand Family
Calbiochem®
Synonyms
rhIL-6
References
References
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
26-October-2007 JSW
Synonyms
rhIL-6
Description
Recombinant, human, interleukin-6 expressed in E. coli. Interleukin-6 is a protein produced by a number of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity. Stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B-cells, T-cells, and hepatocytes. Stimulates growth and inhibits constitutive protein synthesis-independent apoptosis of murine B-cell hybridoma 7TD1. Human sequence IL-6 is active on murine cells. 1 µg is equivalent to 7.0 x 103 WHO units.
Form
Lyophilized
Formulation
Carrier free.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg IL-6
Biological activity
8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
Solubility
Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
