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Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.
Catalogue Number
407617
Brand Family
Calbiochem®
References
References
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Weidmann, B., et al. 1994. Nature370, 434. Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
ED₅₀= 5-10 pg/ml as measured in a cell proliferation assay
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg IL-1β
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
NM9740000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Weidmann, B., et al. 1994. Nature370, 434. Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-May-2008 RFH
Description
Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Interleukin-1 is a protein produced by stimulated macrophages and many other cell types. The following biological activities are attributed to IL-1: activation of T cells resulting in the production of IL-2 and expression of IL-2 receptors; initiation of Acute Phase Response by induction of hepatic protein synthesis; increased proteolysis of muscle proteins, amino acid mobilization, and caloric and oxygen utilization; induction of bone resorption; stimulation of fibroblast proliferation; induction of slow-wave sleep; fever production; induction of prostaglandin and leukotriene production; stimulation of thymocyte proliferation; induction of B cell proliferation and maturation and increased immunoglobulin synthesis; induction of collagenase and other proteinases in synovial fibroblasts and articular chrondrocytes, suggesting a role in pathological states such as arthritis; activation of neutrophils. 1 µg is equivalent to 1.1 x 106 WHO units.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
RTECS
NM9740000
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg IL-1β
Biological activity
ED₅₀= 5-10 pg/ml as measured in a cell proliferation assay
Solubility
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity
Standard Handling
Merck USA index
14, 4993
References
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Weidmann, B., et al. 1994. Nature370, 434. Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.
