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Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. Induces nitric oxide synthase in pancreatic and smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.
Catalogue Number
407615
Brand Family
Calbiochem®
Synonyms
rhIL-1β
References
References
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Kunz, D., et al. 1994. Biochem. J. 304, 337. Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62. Dinarello, C>A> 1991. Blood77, 1627. Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357. Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.
Product Information
CAS number
97599-21-8
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
ED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg IL-1β
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
NM9740000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Kunz, D., et al. 1994. Biochem. J. 304, 337. Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62. Dinarello, C>A> 1991. Blood77, 1627. Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357. Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-May-2008 RFH
Synonyms
rhIL-1β
Description
Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. IL-1β is activated by proteolytic cleavage of the precursor molecule. Increases nitric oxide production in smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Induces nitric oxide synthase in insulin-producing cells. 1 µg of IL-1β corresponds to 1.9 x 105 WHO units.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
CAS number
97599-21-8
RTECS
NM9740000
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg IL-1β
Biological activity
ED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)
Solubility
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity
Standard Handling
Merck USA index
14, 4993
References
Chun, S.Y., et al. 1995. Endocrinology136, 3120. Kunz, D., et al. 1994. Biochem. J. 304, 337. Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62. Dinarello, C>A> 1991. Blood77, 1627. Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357. Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.
