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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

688001 Sigma-AldrichInSolution™ Y-27632 - Calbiochem

Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (K_i = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

InSolution™ Y-27632 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: Rho Kinase Inhibitor VI

Primary Target: p160ROCK

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概述

Replacement Information

重要规格表

Empirical Formula
C₁₄H₂₁N₃O · 2HCl · H₂O

价格及供货情况

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Description
Overview	A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (K_i = 140 nM for p160^ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contractions (IC₅₀ = 700 nM for phenylephrine-induced contractions in aortic strips).
Catalogue Number	688001
Brand Family	Calbiochem®
Synonyms	Rho Kinase Inhibitor VI

References
References	Chitaley, K., et al. 2001. Nat. Med. 7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol. 325, 273. Hirose, M., et al. 1999. J. Cell Biol. 141, 1625. Maekawa, M., et al. 1999. Science 285, 895. Uehata, M., et al. 1997. Nature 389, 990.

Product Information
ATP Competitive	Y
Declaration	Sold under license of PCT Application W098/06,433A1 and under license from Mitsubishi Pharma Tanabe Corporation.
Form	Liquid
Formulation	A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688000) in H₂O.
Hill Formula	C₁₄H₂₁N₃O · 2HCl · H₂O
Chemical formula	C₁₄H₂₁N₃O · 2HCl · H₂O
Reversible	N
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	p160ROCK
Primary Target IC<sub>50</sub>	700 nM against phenylephrine-induced contractions in aortic strips
Primary Target K<sub>i</sub>	140 nM for p160ROCK (ROCK-I)
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	Y

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 20/21/22 Harmful by inhalation, in contact with skin and if swallowed.
S Phrase	S: 36 Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Harmful
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
688001-500UGCN	07790788052386

Documentation

InSolution™ Y-27632 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

InSolution™ Y-27632 - Calbiochem 分析证书

标题	批号
688001	输入批号

参考

参考信息概述
Chitaley, K., et al. 2001. Nat. Med. 7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol. 325, 273. Hirose, M., et al. 1999. J. Cell Biol. 141, 1625. Maekawa, M., et al. 1999. Science 285, 895. Uehata, M., et al. 1997. Nature 389, 990.

引用

标题

James J. Fiordalisi, Patricia J. Keller and Adrienne D. Cox. (2006) PRL Tyrosine Phosphatases Regulate Rho Family GTPases to Promote Invasion and Motility. Cancer Research 66, 3153-3161.

Karen E. Porter, et al. (2004) Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA. Cardiovascular Research 61, 745-755.

Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	16-September-2008 RFH
Synonyms	Rho Kinase Inhibitor VI
Description	A 5 mM (500 µg/296 µl) aqueous solution of Y-27632 (Cat. No. 688000). A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (K_i = 140 nM for p160^ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contractions (IC₅₀ = 700 nM for phenylephrine-induced contractions in aortic strips).
Form	Liquid
Formulation	A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688000) in H₂O.
Intert gas (Yes/No)	Packaged under inert gas
Chemical formula	C₁₄H₂₁N₃O · 2HCl · H₂O
Structure formula
Purity	≥95% by HPLC
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Harmful
References	Chitaley, K., et al. 2001. Nat. Med. 7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol. 325, 273. Hirose, M., et al. 1999. J. Cell Biol. 141, 1625. Maekawa, M., et al. 1999. Science 285, 895. Uehata, M., et al. 1997. Nature 389, 990.
Citation	James J. Fiordalisi, Patricia J. Keller and Adrienne D. Cox. (2006) PRL Tyrosine Phosphatases Regulate Rho Family GTPases to Promote Invasion and Motility. Cancer Research 66, 3153-3161. Karen E. Porter, et al. (2004) Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA. Cardiovascular Research 61, 745-755. Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.

种类

Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Rho Kinase (ROCK) Inhibitors

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688001 Sigma-AldrichInSolution™ Y-27632 - Calbiochem