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IPVH304F0 Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm

Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析

Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
主要应用: 西方蛋白印迹
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IPVH304F0
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      概述

      Replacement Information

      重要规格表

      主要应用
      西方蛋白印迹
      描述
      产品目录编号IPVH304F0
      商名
      • Immobilon
      描述Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm
      背景信息Overview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      参考
      产品信息
      检测方法呈色 放射
      相容的染色剂
      • 考马斯亮蓝
      • 酰胺黑
      • 印度墨水
      • 丽春红
      • 胶体金
      • CPTS
      • 甲苯胺蓝
      • 透射显影
      • Sypro® 宝石红
      过滤器代码IPVH
      过滤器颜色白色
      Quality LevelMQ400
      应用
      应用Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析
      主要应用
      • 西方蛋白印迹
      生物信息
      主结合机制静电，疏水
      BSA吸收性215 µg/cm²
      山羊IgG吸收性294 µg/cm²
      胰岛素吸收性160 µg/cm²
      可湿润性疏水
      理化信息
      孔径0.45 µm
      尺寸
      过滤器表面平纹
      过滤器尺寸26 cm x 26 cm
      材料信息
      化学
      • 疏水性聚偏氟乙烯（PVDF）
      毒性学信息
      GHS安全信息
      安全信息
      产品使用声明
      储存和货运信息
      包装信息
      数量10
      运输信息
      补充信息
      规格
      Global Trade Item Number
      产品目录编号 GTIN
      IPVH304F0 04053252471339

      Documentation

      Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm MSDS

      职位

      物料安全数据表 (MSDS) 

      参考

      参考概述应用
      Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
      Luo S., Wehr N.B., Levine R.L.
      Analytical Biochemistry:350 (2006):233-238  2006

      		Immunoblotting (Western)

      小册子

      标题
      Western Blotting Tools

      技术信息

      标题
      Protein Blotting Handbook: 6th Edition

      数据表

      标题
      Immobilon Transfer Membranes: For superior protein and nucleic acid blots

      用户指南

      标题
      Immobilon -P Blotting Sandwiches
      Immobilon®-P Transfer Membrane User Guide