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Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
Catalogue Number
407255
Brand Family
Calbiochem®
Synonyms
IGFBP-7
References
References
Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612. Nagakubo, D. et al. 2003. J. Immunol.171, 553. Wilson, HM. et al. 2002. Cell Growth Differ.13, 205. Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747. Kato, M.V. 2000. Mol. Med.6, 126. Hwa, V. et al. 1999. Endocr. Rev.20, 761. Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981. Kato, M.V. et al. 1996. Oncogene12, 1361. Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.
Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU per/1 µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612. Nagakubo, D. et al. 2003. J. Immunol.171, 553. Wilson, HM. et al. 2002. Cell Growth Differ.13, 205. Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747. Kato, M.V. 2000. Mol. Med.6, 126. Hwa, V. et al. 1999. Endocr. Rev.20, 761. Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981. Kato, M.V. et al. 1996. Oncogene12, 1361. Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
21-Sepmtember-2011 RFH
Synonyms
IGFBP-7
Description
Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
Form
Solid
Formulation
Lyophilized from 0.2 µm sterile-filtered PBS.
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: <1.0 EU per/1 µg protein
Biological activity
Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
Solubility
Reconstitute to 100 µg/ml in sterile PBS.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612. Nagakubo, D. et al. 2003. J. Immunol.171, 553. Wilson, HM. et al. 2002. Cell Growth Differ.13, 205. Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747. Kato, M.V. 2000. Mol. Med.6, 126. Hwa, V. et al. 1999. Endocr. Rev.20, 761. Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981. Kato, M.V. et al. 1996. Oncogene12, 1361. Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.
