数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

SCR502 Sigma-AldrichHuman, iPS Selection Kit

The Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.

Human, iPS Selection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Key Applications: Immunocytochemistry

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

重要规格表

Key Applications
ICC

Description
Catalogue Number	SCR502
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Human, iPS Selection Kit
Overview	Millipore’s Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC). The Human iPS Selection Kit allows live cell imaging and identification of fully reprogrammed human iPS cells from a heterogeneous population of reprogramming intermediates and enables the live selection of human iPS cells that can be further passaged and expanded for downstream applications. The selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally. The kit consists of the following: (1) two conjugated antibodies that are known markers of human pluripotency, Tra-I-60 and SSEA-4, (2) a negative selection marker (CD13-PE) that is specific to fibroblasts and (3) a cell permeable Hoechst nuclear dye that possesses the dual function of labeling all cells and also distinguishing partially reprogrammed cells (Hoechst Bright) from fully reprogrammed cells (Hoechst Dim) (Chan et al,).
Background Information	Recent findings indicate that fully reprogrammed human iPS cells possess the following characteristics: (1) they downregulate expression of fibroblast marker, CD13 (2) they upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60, (3) they silence the viral transgenes while (4) reactivating endogenous expression of Nanog and (5) they assume a HoeschstDim phenotype. These characteristics have enabled reliable identification of fully reprogrammed human iPS cells from a mixed population of partially reprogrammed cells.

References

Product Information
Components	Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50 μg of 0.25 mg/mL purified mouse IgG antibody Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50 μg of 0.5 mg/mL purified mouse IgM antibody Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50 μg of 1 mg/mL purified mouse IgG antibody Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 15 μL of 1 mg/mL stock solution.
Quality Level	MQ100

Product Information

Components

Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50 μg of 0.25 mg/mL purified mouse IgG antibody
Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50 μg of 0.5 mg/mL purified mouse IgM antibody
Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50 μg of 1 mg/mL purified mouse IgG antibody
Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 15 μL of 1 mg/mL stock solution.

Quality Level

MQ100

Applications
Application	The Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.
Key Applications	Immunocytochemistry

Biological Information
Species Reactivity	Human
Stem Cell Type	Human Embryonic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2-8°C, protected from light in undiluted aliquots.

Packaging Information
Material Size	1 kit
Material Package	Sufficient for 40 tests per antibody

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
SCR502	04053252423338

Documentation

Human, iPS Selection Kit MSDS

职位
物料安全数据表 (MSDS)

Human, iPS Selection Kit 分析证书

标题	批号
3380484	3380484
Human iPS Selection Kit - 2063187	2063187
Human iPS Selection Kit - 2109548	2109548
Human iPS Selection Kit - 2315728	2315728
Human iPS Selection Kit - 2363021	2363021
Human iPS Selection Kit - 2469361	2469361
Human iPS Selection Kit - 1990698	1990698
Human iPS Selection Kit - 2142704	2142704
Human iPS Selection Kit - 2268801	2268801
Human iPS Selection Kit - 2530135	2530135

Accessories

产品目录编号

描述

SCM075

ROCK Inhibitor (Y-27632)

显示价格及库存情况

种类

Life Science Research > Cell Analysis > Cell-based Assays > Cell Characterization Assays

此产品已添加到您的购物车。