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382187
Sigma-AldrichHDAC Inhibitor XXIII, Tubastatin A - Calbiochem
The HDAC Inhibitor XXIII, Tubastatin A controls the biological activity of HDAC. This small molecule/inhibitor is primarily used for Cell Structure applications.
More>>The HDAC Inhibitor XXIII, Tubastatin A controls the biological activity of HDAC. This small molecule/inhibitor is primarily used for Cell Structure applications. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
A cell-permeable carbazolohydroxamate that acts as a highly potent HDAC6-selective inhibitor (IC50 = 15 nM) with much reduced or no activity against other known HDACs. Effectively prevents neuronal cell death (by ≥95% at 10 µM) upon oxidative stress induction by HCA and selectively induces cellular α-tubulin, but not histone H4, hyperacetylation (2.5 to 5 µM) in primary rat cortical neuron cultures.
HDAC Inhibitor XXIII, Tubastatin A - Calbiochem 分析证书
标题
批号
382187
参考
参考信息概述
Butler, K.V., et al. 2010. J. Am. Chem. Soc.132, 10842.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
A cell-permeable carbazolohydroxamate whose zinc-chelating hydroxamic acid moiety and catalytic channel rim-targeting aromatic cap structure render it a highly potent HDAC6-selective inhibitor (IC50 = 15 nM against recombinant human HDAC6) with much reduced or no activity against HDAC8, HDAC1 (IC50 = 0.854 and 16.4 µM, respectively), and other known HDACs (HDAC2/3/4/5/7/9/10/11; IC50 >30 µM). Shown to selectively block HDAC6-, but not HDAC1-, dependent cellular deacetylation as evidenced by its selective induction (2.5 to 5 µM) of α-tubulin, but not histone H4, hyperacetylation in cultured primary rat cortical neurons. Although failing to block cellular glutathione depletion by homocysteic acid (HCA), Tubastatin A nevertheless is reported to prevent neuronal cell death upon oxidative stress induction by HCA in a dose-dependent manner (≥95% protection at 10 µM), presumably by preventing peroxiredoxins deacetylation.
Form
Tan solid
Intert gas (Yes/No)
Packaged under inert gas
Chemical formula
C₂₀H₂₁N₃O₂
Structure formula
Purity
≥95% by HPLC
Solubility
DMSO (10 mg/ml) or Ethanol (250 µg/ml)
Storage
Protect from light
-20°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Butler, K.V., et al. 2010. J. Am. Chem. Soc.132, 10842.
