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368067 Sigma-AldrichGranzyme B Substrate VIII, Colorimetric - CAS 216757-29-8 - Calbiochem

A colorimetric substrate for granzyme B (kcat/Km = 6.6 x 10⁴ M⁻¹s⁻¹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

同义词: Ac-IEPD-pNA

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概述

Replacement Information

价格及供货情况

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Description
Overview	A colorimetric substrate for granzyme B (k_cat/K_m = 6.6 x 10⁴ M^-1s^-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M^-1cm^-1).
Catalogue Number	368067
Brand Family	Calbiochem®
Synonyms	Ac-IEPD-pNA

References
References	Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Product Information
CAS number	216757-29-8
ATP Competitive	N
Form	White lyophilized solid
Hill Formula	C₂₈H₃₈N₆O₁₁
Chemical formula	C₂₈H₃₈N₆O₁₁
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Primary Target	Colorimetric substrate for granzyme B
Primary Target IC<sub>50</sub>	kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	N
Peptide Sequence	Ac-Ile-Glu-Pro-Asp-pNA

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
368067-5MGCN	04055977192186

Documentation

Granzyme B Substrate VIII, Colorimetric - CAS 216757-29-8 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Granzyme B Substrate VIII, Colorimetric - CAS 216757-29-8 - Calbiochem 分析证书

标题	批号
368067	输入批号

参考

参考信息概述
Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	07-July-2008 RFH
Synonyms	Ac-IEPD-pNA
Description	A colorimetric substrate for granzyme B (k_cat/K_m = 66,600 M_-1s_-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M_-1cm_-1).
Form	White lyophilized solid
Intert gas (Yes/No)	Packaged under inert gas
Recommended reaction conditions	0.25 µM substrate, 100 mM HEPES buffer, pH 7.5, and 10 mM DTT. Note: optimal enzyme concentration and incubation time should be determined empirically for each assay.
CAS number	216757-29-8
Chemical formula	C₂₈H₃₈N₆O₁₁
Peptide Sequence	Ac-Ile-Glu-Pro-Asp-pNA
Purity	≥95% by HPLC
Solubility	DMSO (5 mg/ml)
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity	Standard Handling
References	Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

种类

Life Science Research > Inhibitors and Biochemicals

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