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GDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.
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MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
Catalogue Number
345749
Brand Family
Calbiochem®
Synonyms
Myostatin Propeptide
References
References
Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144. Thies, R.S. et al. 2001. Growth Factors18, 251. Chang, H. et al. 2002. Endocrine Reviews23, 787. McPherron, A.C. et al. 1997. Nature387, 83.
GDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.
Biological Information
Biological activity
ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
Purity
≥90% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144. Thies, R.S. et al. 2001. Growth Factors18, 251. Chang, H. et al. 2002. Endocrine Reviews23, 787. McPherron, A.C. et al. 1997. Nature387, 83.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-June-2011 RFH
Synonyms
Myostatin Propeptide
Description
Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered PBS, BSA.
Purity
≥90% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Biological activity
ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
Solubility
Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144. Thies, R.S. et al. 2001. Growth Factors18, 251. Chang, H. et al. 2002. Endocrine Reviews23, 787. McPherron, A.C. et al. 1997. Nature387, 83.