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Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Supplied as a crude preparation of soluble furin obtained from cytoplasmic extracts. Furin is a type I membrane protein that processes stromelysin-3 (MMP-11) and MT1-MMP. It cleaves pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins.
This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.
Catalogue Number
344932
Brand Family
Calbiochem®
References
References
Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224. Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326. Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211. Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953. Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.
Product Information
Unit of Definition
One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.
Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224. Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326. Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211. Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953. Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
21-January-2008 JSW
Description
Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.
Form
Liquid
Formulation
In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.
Unit definition
One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224. Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326. Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211. Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953. Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.
