Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
A rapid and sensitive method for detecting protein and nucleic acids fractionated by PAGE. Provides gels with clear backgrounds and sharp bands. Silver staining is about 100 times more sensitive than the standard Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA.
FASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+15°C to +30°C
Storage Conditions
Upon arrival store entire kit contents at room temperature (20°C).
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Silver stain, developer, sensitizers I and II, and a user protocol. Note: one kit provides reagents sufficient for staining 25 mini gels.
Upon arrival store entire kit contents at room temperature (20°C).
Intended use
FASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
• Fixative I: Mix 30 ml 100% ethanol, 10 ml glacial acetic acid, and 60 ml ultra-pure water to obtain 100 ml Fixative I.
• Fixative II: Mix 10 ml 100% ethanol and 90 ml ultra-pure water to obtain 100 ml Fixative II.
• Sensitizer: Mix 5 ml silver stain, 65 µl sensitizer-I, and 45 ml ultra-pure water to obtain 50 ml Sensitizer.
• Developer: Mix 2.5 g developer, 32.5 µl sensitizer-I, 32.5 µl sensitizer-II, and 50 ml ultra-pure water to obtain 50 ml Developer.
• Stopper: Mix 11 ml glacial acetic acid and 39 ml ultra-pure water to obtain 50 ml Stopper.
Detailed protocol
1. After electrophoresis, fix the gel in 50 ml of the freshly prepared Fixative I. Use highly purified deionized water to make the solution. For isoelectric focusing gels, fix the gel first in 20% TCA for 60 min.
2. Fix the gel for 30 min to 3 h depending upon its thickness. For mini gels, (8 x 10 cm), 30 min is sufficient.
3. Wash twice, 10 min each, in 50 ml of the freshly prepared Fixative II.
4. Wash three times, 10 min each, in ultra-pure water. During washing Steps 2 and 3, use generous amounts of water (100 ml per 8 x 10 cm mini gels) and use gentle rocking or agitation.
5. Soak the gel in 50 ml of freshly prepared Sensitizer for 30 min, with gentle rocking of the gel, depending upon the thickness of the gel.
6. Rinse the gel only for 10-20 s with 50 ml of ultra-pure water.
7. Soak the gel in 50 ml of freshly prepared Developer. Gently rock the gel until bands are visible. Band intensity will develop quickly.
8. As soon as band intensity reaches an acceptable level, stop color development by adding 5 ml of the freshly prepared Stopper. Gently rock the gel for 10 min. Store the gel in ultra-pure water for subsequent analysis.
Sensitivity Notes
The lower detection limit of FASTsilver™ gel stain is 1 ng/band for proteins and nucleic acids.
Registered Trademarks
Calbiochem® is a registered trademark of EMD Chemicals, Inc. Interactive Pathways™ is a trademark of EMD Chemicals, Inc. FASTsilver™ is a trademark of Geno Technology, Inc.
