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324726 Sigma-AldrichEndoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli

Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides. It is not active above pH 6.0.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Endo-β-N-acetylglucosaminidase F2, Endo F2

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概述

Replacement Information

价格及供货情况

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Description
Overview	Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
Overview	Note: 1 mU = 1 milliunit.
Catalogue Number	324726
Brand Family	Calbiochem®
Synonyms	Endo-β-N-acetylglucosaminidase F2, Endo F2

References
References	Reddy, A., et al. 1998. Glycobiology 8, 633. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Product Information
Activity	≥5 units/ml
Unit of Definition	One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
EC number	3.2.1.96
Form	Liquid
Formulation	In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
Quality Level	MQ100

Applications

Biological Information
Specific Activity	≥20 units/mg protein

Physicochemical Information
Contaminants	N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
324726-200MIUCN	04055977216004

Documentation

Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli MSDS

职位
物料安全数据表 (MSDS)

Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli 分析证书

标题	批号
324726	输入批号

参考

参考信息概述
Reddy, A., et al. 1998. Glycobiology 8, 633. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	11-September-2007 RFH
Synonyms	Endo-β-N-acetylglucosaminidase F2, Endo F2
Description	Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore, is more suitable for deglycosylation of native proteins.
Form	Liquid
Formulation	In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
Recommended reaction conditions	50 mM sodium acetate buffer, pH 4.5. (Note: enzyme is not active above pH 6.0)
EC number	3.2.1.96
Contaminants	N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
Specific activity	≥20 units/mg protein
Activity	≥5 units/ml
Unit definition	One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Reddy, A., et al. 1998. Glycobiology 8, 633. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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