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345811 Sigma-AldrichEndo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

价格及供货情况

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Description
Overview	Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Catalogue Number	345811
Brand Family	Calbiochem®

References
References	Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709. Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365. Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586. Scudder, P., et al. 1983. Biochem. J. 213, 485.

Product Information
Activity	≥14 U/ml
Unit of Definition	One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
EC number	3.2.1.103
Form	Liquid
Formulation	Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
Quality Level	MQ100

Applications

Biological Information
Specific Activity	≥140 U/mg protein

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
345811-50ULCN	04055977214970

Documentation

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli MSDS

职位
物料安全数据表 (MSDS)

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli 分析证书

标题	批号
345811	输入批号

参考

参考信息概述
Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709. Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365. Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586. Scudder, P., et al. 1983. Biochem. J. 213, 485.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	22-June-2007 JSW
Description	Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Form	Liquid
Formulation	Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditions	Activity Assay Required Reagents • 5X Reaction Buffer: 250 mM sodium phosphate, pH 5.8 Assay For Glycoproteins 1. Add up to 100 µg glycoprotein to a clean tube. 2. Add 4 µl 5X Reaction Buffer. 3. Add H₂O to a total volume of 19 µl. 4. Add 1 µl endo-β-galactosidase. 5. Incubate at 37°C for 2 h. For Oligosaccharides Follow the assay as outlined above in steps 1-4. Incubate at 37°C for several h to several days, depending on the substrate.
EC number	3.2.1.103
Specific activity	≥140 U/mg protein
Activity	≥14 U/ml
Unit definition	One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
Toxicity	Standard Handling
References	Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709. Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365. Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586. Scudder, P., et al. 1983. Biochem. J. 213, 485.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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