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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PMEF-NX Sigma-AldrichEmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Irradiated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

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概述

Replacement Information

特卖

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重要规格表

Key Applications
Cult

Description
Catalogue Number	PMEF-NX
Trade Name	Embryomax Specialty Media
Description	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Overview	Primary Mouse Embryo Fibroblasts, neo resistant, Strain FVB, are mitotically inactivated by treatment with irradiation and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin. Plating MEF Feeder Cells Procedure: 1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution. 2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes. 3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium. 4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide 5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Background Information	The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

References

Product Information
Quality Level	MQ100

Applications
Application	PMEF, Neo Resistant, Strain FVB, Irradiated, Passage 3
Key Applications	Stem Cell Culture

Biological Information
Stem Cell Type	Mouse Embryonic Stem Cells PMEFs Induced Pluripotent Stem Cells
Cell Line Type	Primary Mouse Embryo Fibroblasts

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Vials should be stored in the vapor phase of liquid nitrogen.

Packaging Information
Material Size	5 vials
Material Package	5-6x10⁶ ea

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PMEF-NX	04053252923678

Documentation

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS

职位
物料安全数据表 (MSDS)

EmbryoMax® Primary Mouse Embryonic Fibroblasts 分析证书

标题	批号
EmbryoMax#174; PMEF P3 Strain Neo, Irradiated - RD1304001-1	RD1304001-1
EmbryoMax#174; PMEF P3 Strain Neo, Irradiated - RD1304014-1	RD1304014-1
EmbryoMax® PMEF P3 -2503243-1	2503243-1
EmbryoMax® PMEF P3 -2775538-1	2775538-1
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3085994-2	3085994-2
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3614409-1	3614409-1
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3614409-2	3614409-2
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3670243-2	3670243-2
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3833628-2	3833628-2
EmbryoMax® PMEF P3 Strain Neo, Irradiated - 3946855-1	3946855-1

技术信息

标题
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

种类

Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells

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