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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PMEF-NL-P1 Sigma-AldrichEmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

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概述

Replacement Information

特卖

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重要规格表

Key Applications
Cult

Description
Catalogue Number	PMEF-NL-P1
Trade Name	Embryomax Specialty Media
Description	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Overview	PMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
Alternate Names	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Background Information	The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

References

Product Information
Presentation	Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Quality Level	MQ100

Applications
Application	PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1
Key Applications	Stem Cell Culture

Biological Information
Stem Cell Type	Mouse Embryonic Stem Cells PMEFs Induced Pluripotent Stem Cells
Cell Line Type	Primary Mouse Embryo Fibroblasts

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Packaging Information
Material Size	1 vial
Material Package	1x10⁶ cells

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PMEF-NL-P1	04053252851810

Documentation

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS

职位
物料安全数据表 (MSDS)

EmbryoMax® Primary Mouse Embryonic Fibroblasts 分析证书

标题	批号
EmbryoMax® PMEF P1 Neo Resistant, Untreated - 3506189	3506189
EmbryoMax® PMEF P1 Neo Resistant, Untreated - 4119053	4119053

技术信息

标题
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

Alternative Format

产品目录编号

描述

PMEF-NL

EmbryoMax® Primary Mouse Embryonic Fibroblasts

显示价格及库存情况

种类

Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells

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