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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PMEF-HL Sigma-AldrichEmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Hygro Resistant, Strain C57/BL6, Not Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子
技术信息
数据表
Protocols

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概述

Replacement Information

特卖

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重要规格表

Key Applications
Cult

Description
Catalogue Number	PMEF-HL
Trade Name	Embryomax Specialty Media
Description	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Overview	Primary Mouse Embryo Fibroblasts, hygromycin resistant, strain C57/BL6, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to hygromycin. The cells are provided at passage 3 and can be further expanded before freezing for later use. Plating MEF Feeder Cells Procedure: 1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution. 2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes. 3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium. 4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide 5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names	MEF Feeder Cells, hygro PMEFs, hygro MEFs, MEFs, Mouse Feeder Cells
Background Information	The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

References

Product Information
Presentation	The cells are provided frozen into individual vials, containing approximately 5-6 x 10⁶ fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3.
Quality Level	MQ100

Applications
Application	PMEF, Hygro Resistant, Strain C57/BL6, Not Treated, Passage 3
Key Applications	Stem Cell Culture

Biological Information
Stem Cell Type	Mouse Embryonic Stem Cells PMEFs Induced Pluripotent Stem Cells
Cell Line Type	Primary Mouse Embryo Fibroblasts

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Packaging Information
Material Size	5 vials
Material Package	5-6x10⁶ ea

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PMEF-HL	04053252615238

Documentation

Protocols

Title
Plating PMEF Feeder Cells Protocol

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS

职位
物料安全数据表 (MSDS)

EmbryoMax® Primary Mouse Embryonic Fibroblasts 分析证书

标题	批号
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 2028739-1	2028739-1
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 00202-2	00202-2
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 00517-2	00517-2
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 00628-2	00628-2
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 2021874	2021874
EmbryoMax#174; PMEF Cells Hygro Resistant, Untreated - 2061134-1	2061134-1
EmbryoMax® PMEF Cells -2343675-1	2343675-1
EmbryoMax® PMEF Cells -2440295-1	2440295-1
EmbryoMax® PMEF Cells -2440295-3	2440295-3
EmbryoMax® PMEF Cells -2665880-1	2665880-1

小册子

标题
Murine Embryonic Stem Cell Culture Procedures & Protocols

技术信息

标题
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

数据表

标题
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

种类

Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells

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