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PMEF-CFL Sigma-AldrichEmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Not Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子
技术信息
数据表
Protocols

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概述

Replacement Information

特卖

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重要规格表

Key Applications
Cult

Description
Catalogue Number	PMEF-CFL
Trade Name	Embryomax Specialty Media
Description	EmbryoMax® Primary Mouse Embryonic Fibroblasts
Overview	Primary Mouse Embryo Fibroblasts, Strain CF1, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use. Plating MEF Feeder Cells Procedure: 1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution. 2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes. 3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium. 4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide 5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names	MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
Background Information	The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

References

Product Information
HS Code	3821 00 00
Presentation	The cells are provided frozen into individual vials, containing approximately 5-6 x 10⁶ fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3. See ES Stem Cell Manual for complete culture and medias for PMEF cells.
Quality Level	MQ100

Applications
Application	PMEF, Strain CF1, Not Treated, Passage 3
Key Applications	Stem Cell Culture

Biological Information
Stem Cell Type	Human Embryonic Stem Cells Mouse Embryonic Stem Cells PMEFs Induced Pluripotent Stem Cells
Cell Line Type	Primary Mouse Embryo Fibroblasts

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Packaging Information
Material Size	5 vials
Material Package	5-6x10⁶ ea

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PMEF-CFL	04053252552045

Documentation

Protocols

Title
Plating PMEF Feeder Cells Protocol

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS

职位
物料安全数据表 (MSDS)

EmbryoMax® Primary Mouse Embryonic Fibroblasts 分析证书

标题	批号
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 2060019-1	2060019-1
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 2065679-1	2065679-1
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 01030-2	01030-2
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 01030-5	01030-5
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 10109-4	10109-4
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 1970506	1970506
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 1981675	1981675
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 1983305	1983305
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 1990100	1990100
EmbryoMax#174; PMEF Cells Strain CF-1, Untreated - 2013717	2013717

小册子

标题
Murine Embryonic Stem Cell Culture Procedures & Protocols

技术信息

标题
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

数据表

标题
STEMCCA Lentivirus Reprogramming Kits

Alternative Format

产品目录编号

描述

PMEF-CFL-P1

EmbryoMax® Primary Mouse Embryonic Fibroblasts

显示价格及库存情况

种类

Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells

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