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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

324699 Sigma-AldrichElastase Substrate IV, Colorimetric - Calbiochem

A specific substrate for pancreatic elastase.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

价格及供货情况

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Description
Overview	A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
Catalogue Number	324699
Brand Family	Calbiochem®

References
References	Largman, L. 1983. Biochemistry 22, 3763. Del Mar, E.G., et al. 1980. Biochemistry 19, 468.

Product Information
ATP Competitive	N
Form	White to off-white lyopilized solid
Formulation	45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Hill Formula	C₂₅H₃₄N₆O₉
Chemical formula	C₂₅H₃₄N₆O₉
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Primary Target	A specific substrate for pancreatic elastase
Primary Target IC<sub>50</sub>	km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	N
Peptide Sequence	Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Moisture	Protect from moisture
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
324699-5MGCN	04055977215915

Documentation

Elastase Substrate IV, Colorimetric - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Elastase Substrate IV, Colorimetric - Calbiochem 分析证书

标题	批号
324699	输入批号

参考

参考信息概述
Largman, L. 1983. Biochemistry 22, 3763. Del Mar, E.G., et al. 1980. Biochemistry 19, 468.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-June-2008 RFH
Description	A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm. Suggested protocol: see Largman, C. (1983)
Form	White to off-white lyopilized solid
Formulation	45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Chemical formula	C₂₅H₃₄N₆O₉
Peptide Sequence	Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
Purity	≥95% by HPLC
Solubility	Ethanol (1 mg/ml) or H₂O
Storage	Protect from moisture Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Toxicity	Standard Handling
References	Largman, L. 1983. Biochemistry 22, 3763. Del Mar, E.G., et al. 1980. Biochemistry 19, 468.

种类

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Protease Substrates

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