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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

324681 Sigma-AldrichElastase, Human Neutrophil

Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans

Elastase, Human Neutrophil MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: PMN Elastase

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概述

Replacement Information

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
324681-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	玻璃瓶	100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹
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Description
Overview	Native elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
Catalogue Number	324681
Brand Family	Calbiochem®
Synonyms	PMN Elastase

References
References	Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855. Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691. Selak, M.A. 1992. Thromb. Haemost. 68, 570. Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.

Product Information
CAS number	9004-06-2
Unit of Definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=454454">Cat. No. 454454</a>) per min at 25°C, pH 8.0.
EC number	3.4.21.37
Form	Lyophilized solid
Formulation	Salt-free.
Quality Level	MQ200

Applications

Biological Information
Purity	Major band by SDS-PAGE
Source	Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity	≥20 units/mg protein.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	JX7069000

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
324681-100UGCN	07790788059583
324681-50UGCN	04055977196825

Documentation

Elastase, Human Neutrophil MSDS

职位
物料安全数据表 (MSDS)

Elastase, Human Neutrophil 分析证书

标题	批号
324681	输入批号

参考

参考信息概述
Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855. Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691. Selak, M.A. 1992. Thromb. Haemost. 68, 570. Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.

引用

标题

Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.

Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.

Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-May-2008 RFH
Synonyms	PMN Elastase
Description	Native elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.
Form	Lyophilized solid
Formulation	Salt-free.
Recommended reaction conditions	Human Neutrophil Elastase Protocol 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
Source	Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number	9004-06-2
RTECS	JX7069000
EC number	3.4.21.37
Purity	Major band by SDS-PAGE
Specific activity	≥20 units/mg protein.
Unit definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
Solubility	Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
Toxicity	Standard Handling
Merck USA index	14, 3535
References	Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855. Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691. Selak, M.A. 1992. Thromb. Haemost. 68, 570. Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
Citation	Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660. Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421. Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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