Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recombinant, human E-selectin consisting of 535 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is transiently expressed on vascular endothelial cell surfaces where it is able to bind to ligands such as SLex found on leukocytes. This initial binding event is followed by a second interaction involving ICAM and VCAM-1 that leads to vascular penetration and leukocyte invasion into the extracellular matrix tissue.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-September-2008 RFH
Synonyms
CD62E, ELAM-1
Description
Recombinant, human E-selectin consisting of 535 amino acids minus the transmembrane and cytoplasmic domains expressed in CHO cells. This glycoprotein is transiently expressed on vascular endothelial cell surfaces where it is able to bind to ligands, such as sialyl Lewis x found on leukocytes. This initial binding event is followed by a second interaction involving ICAM and VCAM-1 that leads to vascular penetration and leukocyte invasion into the extracellular matrix tissue.
Form
Lyophilized
Formulation
Lyophilized from Dulbecco's PBS with stabilizer.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: <1 EU/µg of E-Selectin
Biological activity
Shown to bind to E-selectin ligands on U937 cells.
Solubility
Reconstitute to 1 mg/ml with sterile H₂O.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
