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371206 Dkk-1, His•Tag®, Mouse, Recombinant

Dkk-1, His•Tag®, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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371206
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概述

Replacement Information

重要规格表

Purity
≥95% by SDS-PAGE

价格及供货情况

产品目录编号 库存情况包装 数量 / 包装 价格 数量
371206-10UGCN
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      Description
      OverviewRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
      Catalogue Number371206
      Brand Family Calbiochem®
      References
      ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
      Li, J. et al. 2006. Bone 36, 754.
      Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Verani, R. et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M. et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Glinka, A. et al. 1998. Nature 391, 357.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
      Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Regulatory Review
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      371206-10UGCN 04055977191783

      Documentation

      Dkk-1, His•Tag®, Mouse, Recombinant MSDS

      职位

      物料安全数据表 (MSDS) 

      Dkk-1, His•Tag®, Mouse, Recombinant 分析证书

      标题批号
      371206

      参考

      参考信息概述
      Pinzone, J.J. et al. 2009. Blood 113, 517.
      Li, J. et al. 2006. Bone 36, 754.
      Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Verani, R. et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M. et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Glinka, A. et al. 1998. Nature 391, 357.
      数据表

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2011 RFH
      DescriptionRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
      Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
      SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Regulatory Review
      ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
      Li, J. et al. 2006. Bone 36, 754.
      Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Verani, R. et al. 2006. J. Neurochem. 101, 242.
      Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
      Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
      Adamska, M. et al. 2004. Dev. Biol. 272, 134.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Glinka, A. et al. 1998. Nature 391, 357.