Millipore Sigma Vibrant Logo

317640 Dipeptidyl Peptidase IV (CD26), Porcine Kidney

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: CD26, DPPIV

View Products on Sigmaaldrich.com
317640
查看价格及供货情况

概述

Replacement Information

价格及供货情况

产品目录编号 库存情况包装 数量 / 包装 价格 数量
317640-250MIUCN
正在查询供货情况......
—
目前缺货 目前缺货
有货 
停产
少量现货
供货情况有待确认
    其余：另行通知
      其余：另行通知
      另行通知
      联系客户服务
      Contact Customer Service

      		 塑胶安瓿;塑胶针药瓶 250 miu
      正在检索价格......
      无法检索价格。
      最小订购数量为倍数
      Maximum Quantity is
      以订单确认为准 更多信息
      您节约了 ()。
       
      —
      Request Pricing
      		 —
      Description
      OverviewNative, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
      Catalogue Number317640
      Brand Family Calbiochem®
      SynonymsCD26, DPPIV
      References
      ReferencesBar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
      EC number3.4.14.5
      FormLiquid
      FormulationIn 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥35 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsDilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      317640-250MIUCN 04055977216257

      Documentation

      Dipeptidyl Peptidase IV (CD26), Porcine Kidney MSDS

      职位

      物料安全数据表 (MSDS) 

      Dipeptidyl Peptidase IV (CD26), Porcine Kidney 分析证书

      标题批号
      317640

      参考

      参考信息概述
      Bar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.
      数据表

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      SynonymsCD26, DPPIV
      DescriptionNative, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
      FormLiquid
      FormulationIn 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Activity Assay The specific activity can be assayed using the synthetic substrate, 7-(Gly-Pro)-amino-4-methylcoumarinamide, and the cleavage product, 7-amino-4-methylcourmain (AMC). Both are fluorescent, but the excitation/emission spectra are different; at an excitation/emission setting of 380 nm/460 nm, only the AMC product is measured. For quantitative measurement an AMC standard curve should be included; the recommended range is 100-800 pmol. Alternatively, the activity can be measured using the colorimetric substrate, H-Gly-Pro-pNA. The activity is then monitored at 405 nm over a period of time. A standard curve can be generated using 4-nitroaniline in the range of 5-100 nmol.
      EC number3.4.14.5
      Purity≥90% by SDS-PAGE
      Specific activity≥35 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsDilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
      Toxicity Standard Handling
      ReferencesBar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.