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数量	产品目录编号	订购说明	数量 / 包装	公开价
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309800 Sigma-AldrichDihydroethidium - CAS 38483-26-0 - Calbiochem

A cell-permeable, chemically-reduced ethidium derivative.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

Recommended Products

概述

Replacement Information

重要规格表

CAS #	Empirical Formula
38483-26-0	C₂₁H₂₁N₃

价格及供货情况

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Description
Overview	A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
Catalogue Number	309800
Brand Family	Calbiochem®
Synonyms	2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

References
References	Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100. Mancini, M., et al. 1997. J. Cell Biol. 138, 449. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253. Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187. Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117. Perticarari, S., et al. 1991. Cytometry 12, 687. Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69. Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.

References

Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.

Product Information
CAS number	38483-26-0
ATP Competitive	N
Form	Pink solid
Hill Formula	C₂₁H₂₁N₃
Chemical formula	C₂₁H₂₁N₃
Reversible	N
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	Useful fluorogenic probe for the detection of reactive oxygen species (ROS)
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	Y

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	SF7935000

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
309800-25MGCN	04055977216806

Documentation

Dihydroethidium - CAS 38483-26-0 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Dihydroethidium - CAS 38483-26-0 - Calbiochem 分析证书

标题	批号
309800	输入批号

参考

参考信息概述
Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100. Mancini, M., et al. 1997. J. Cell Biol. 138, 449. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253. Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187. Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117. Perticarari, S., et al. 1991. Cytometry 12, 687. Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69. Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-June-2008 RFH
Synonyms	2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
Description	A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
Form	Pink solid
Intert gas (Yes/No)	Packaged under inert gas
CAS number	38483-26-0
RTECS	SF7935000
Chemical formula	C₂₁H₂₁N₃
Structure formula
Purity	≥95% by HPLC
Solubility	DMF or DMSO (20 mg/ml)
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity	Irritant
References	Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100. Mancini, M., et al. 1997. J. Cell Biol. 138, 449. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253. Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187. Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117. Perticarari, S., et al. 1991. Cytometry 12, 687. Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69. Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.

种类

Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > Superoxide and Hydrogen Peroxide Probes

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