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Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.
Catalogue Number
260913
Brand Family
Calbiochem®
Synonyms
Deoxyribonuclease I
References
References
Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481. Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.
Product Information
CAS number
9003-98-9
Activity
≥60,000 Dornase units/mg dry weight.
Unit of Definition
One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
EC number
3.1.21.1
Form
Lyophilized
Hygroscopic
Hygroscopic
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
RF0750000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Moisture
Protect from moisture
Hygroscopic
Hygroscopic
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481. Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
30-April-2008 RFH
Synonyms
Deoxyribonuclease I
Description
Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
Form
Lyophilized
CAS number
9003-98-9
RTECS
RF0750000
EC number
3.1.21.1
Activity
≥60,000 Dornase units/mg dry weight.
Unit definition
One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
Solubility
Sodium Acetate Buffer or H₂O
Storage
Protect from moisture
-20°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
Merck USA index
14, 2906
References
Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481. Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.
